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Climate change and urchin fertilisation and the effect on the growth rate of juvenile Abatus sp.

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Ho, M.
Publication_Date: 20130321
Title: Climate change and urchin fertilisation and the effect on the growth rate of juvenile Abatus sp.
Edition:
Geospatial_Data_Presentation_Form:
Series_Information:
Series_Name: CAASM Metadata
Issue_Identification:
Publication_Information:
Publication_Place:
Publisher: Australian Antarctic Data Centre
Other_Citation_Details:
Online_Linkage: https://data.aad.gov.au/aadc/metadata/metadata_redirect.cfm?md=/AMD/AU/AAS_3134_urchins_climate_change
Description:
Abstract:
The effect of pH, temperature and sperm concentration on the fertilisation of Sterechinus neumayeri was investigated. Adult Sterechinus neumayeri were collected from Ellis Fjord Narrows between December and January 2011-12 and held in the Ecotox Field Aquarium Module until used. Between 3-4 male and female individuals were spawned using 0.5M KCl and gametes were collected separately before being fertilised in treatment. The data set shows the percentage of fertilised and non-fertilised eggs of Sterechinus neumayeri scored at 20h post-fertilisation. Eggs were fertilised in various combinations of pH, temperature and sperm concentration treatments (pH: 8.0 (Control), 7.8 and 7.6; Temperature: 1 degrees C (Control), 3 degrees C and 5 degrees C; Sperm concentration (sperm:egg ratio): 1000:1 (Control), 750:1, 250: 1, 50:1 and 5:1). At 20h post fertilisation, 5 ml aliquot was removed from fertilisation vials and eggs were counted and determined if they were fertilised or not. Seawater parameters of treatments were measured at the start and end of the experiment. Detailed information of the spreadsheets are as follows: Seawater Parameters column headings: Temperature - measured in degrees C , shows the temperature treatments used pH - shows the pH levels used Subheading pH - pH level measured for the day using NIST certified buffers Subheading MV - pH level measured for the day in millivolts Subheading Total pH - total pH level in seawater obtained from MV measurements Subheading Temp - temperature of seawater measured for the day 1 deg C column headings: Experiment - number of experiments pH - shows the pH for each treatment Sperm Concentration - shows the sperm concentration used for each treatment in a egg:sperm ratio Rep - shows the number of replicates per experiment Unfertilised eggs - eggs without visible fertilisation envelope and no cleavage after 20h Fertilised eggs - eggs with visible fertilisation envelope and/or cleavage after 20h Fertilised deformed eggs - eggs with visible fertilisation envelope but deformed Total eggs - total eggs scored (whether fertilised or unfertilised) % Fertilised - fertilised eggs (deformed and non-deformed)/Total eggs 3 deg C and 5 deg C have the same column headings as 1 deg C. AAS3134 Abatus sp Growth Experiment Davis 2011-12: The effect of pH and temperature on the growth rate of juvenile Abatus ingens and Abatus shackletoni were investigated. Adult Abatus were collected off Airport Beach in waters 4-5m depth. Data set shows the growth rate of juveniles of Abatus ingens and Abatus shackletoni after a 4-week exposure to various combinations of pH and temperature. Juveniles of each species was removed from maternal pouches and photographed on the oral side before being exposed to combinations of pH (8.0 (Control), 7.8 and 7.6) and temperature (-1 degrees C (Control) and 1 degrees C) levels. They were incubated in treatments for 4 weeks before being removed and rephotographed. The lengths of 10 spines per juvenile were measured in the pre- and post-experiment photographs using ImageJ and the difference calculated to get a growth rate per juvenile. Seawater parameters of treatments were measured at the beginning of the experiment and subsequently once a day until the end of the experiment. Detailed information of the spreadsheets are as follows: A ingens (pre-exp) i.e. juvenile Abatus ingens spine lengths measured before exposure to experimental treatments. Column headings are: Spine number and length (mm): Length of each spine (1 - 10) measured per juvenile in mm. R1 - R12: Number of juveniles A ingens (post-exp) i.e. juvenile Abatus ingens spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above. A shackletoni (pre-exp) i.e. juvenile Abatus shackletoni spine lengths measured before exposure to experimental treatments. Column headings are identical to the above. A shackletoni (post-exp) i.e. juvenile Abatus shackletoni spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above. 2011-12 Aquarium pH and temp main headings show different treatment parameters. Column sub-headings are: Date - Date of measured seawater parameters Salinity - salinity of seawater measured Ppm - Amount of CO2 gas pumped into water recorded in parts per million pH - measured pH of seawater using NIST-certified buffers MV - pH of seawater recorded in millivolts Total pH - total pH of seawater derived from MV Temp - Temperature of seawater measured in degrees C.
Purpose:
Not Available
Supplemental_Information:
Not Available
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20111201
Ending_Date: 20120228
Currentness_Reference:Unknown
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20120101
Ending_Date: 20120315
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 77.8
East_Bounding_Coordinate: 78.0
North_Bounding_Coordinate: -68.3
South_Bounding_Coordinate: -68.8
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: ANCILLARY KEYWORDS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ECHINODERMS
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > MOLLUSKS > BIVALVES
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > BENTHIC HABITAT
Theme_Keyword: EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC CHEMISTRY > CARBON AND HYDROCARBON COMPOUNDS > CARBON DIOXIDE
Theme_Keyword: FIELD SURVEYS
Theme_Keyword: LABORATORY
Theme_Keyword: FIELD INVESTIGATION
Theme_Keyword: Ph
Theme_Keyword: Temperature
Theme_Keyword: Sperm Concentration
Theme_Keyword: Fertilisation
Theme_Keyword: Eggs
Theme_Keyword: BIOTA
Theme_Keyword: ENVIRONMENT
Theme_Keyword: OCEANS
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: OCEAN > SOUTHERN OCEAN
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: These data are publicly available for download from the provided URL.
Use_Constraints:
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_3134_urchins_climate_change when using these data.
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: MELANIE HO
Contact_Position: INVESTIGATOR
Contact_Position: TECHNICAL CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: University of Sydney
Address: Department of Anatomy and Histology F13
City: Sydney
State_or_Province: NSW
Postal_Code: 2006
Country: Australia
Contact_Electronic_Mail_Address: mel@anatomy.usyd.edu.au
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Not Available
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: AU/AADC > Australian Antarctic Data Centre, Australia
Contact_Person: DATA OFFICER AADC
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +61 3 6232 3244
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: metadata@aad.gov.au
Resource_Description: AAS_3134_urchins_climate_change
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Word, Excel
Transfer_Size: 170 kb
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/portal/download_file.c...
Access_Instructions:
Download point for the data
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
https://secure3.aad.gov.au/proms/public/projects/r...
Access_Instructions:
Public information for AAS project 3134
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/metadata/citation.cfm?...
Access_Instructions:
Citation reference for this metadata record and dataset
Fees: Free
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Metadata_Reference_Information:
Metadata_Date: 20121109
Metadata_Review_Date: 20130813
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: DAVE J. CONNELL
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +61 3 6232 3244
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: dave.connell@aad.gov.au
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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