Enzyme Production by Psychrophilic Bacteria

Project Description
The objective of this project is to continue the study of Antarctic
bacteria able to produce exoenzymes with high activity at low
temperatures and evaluate their potential industrial application. In
addition, owing to the ecological relevance of these bacterial
activities, we decided to make a broad study of the ... bacterial
communities from which these strains were isolated. Screenings of
psychrophilic bacteria will be made using selective culture media (in
Petri dishes or liquid cultures) that permit us to detect the desired
enzymatic activities (proteases, amylases, lipases, etc). Strains
showing the desired activity will be studied to determine their
psychrophilic character and to optimise the extracellular enzymes
production under different culture. Subsequently, purification and
characterisation of the enzymes showing the biotechnological
potential will be performed. Taxonomic studies will be made by
sequencing 16S rARN gene and bacterial communities will be analyzed
using denaturing gradient gel electrophoresis (DGGE) and restriction
fragments length polymorphism (RFLP). Due to the interest showed by
many biotechnological industries in the metabolic capabilities of
these extremophilic microorganisms agreements are being established in
order to develop studies about the industrial application of the
genome sequence and the products of these isolated strains.