Project Description
Many of the mutagenic or lethal effects of ionizing radiation can be traced to structural and chemical modification of cellular DNA through double-strand breaks (DSB) and clustered lesions. Although the development of mechanistic models of radiation damage in DNA has reached a high level of sophistication, further refinements are needed to understand fully the underlying mechanisms in particular on a molecular level. The proposed studies are designed to provide, in a comprehensive manner, missing information about the molecular pathways that lead from initial deposition of radiative energy to the formation of double strand breaks and lesions in DNA.