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Nella Dan: SIBEX II Cruise Phytoplankton Data
Entry ID: SIBEX_II_phyto


Summary
Abstract: This dataset contains results from the Second International BIOMASS Experiment II (SIBEX II) cruise of the Nella Dan, taken in January 1985. This cruise was the fourth cruise in a series of six. Phytoplankton samples were taken off Antarctica in the Australian sector (Mawson to Davis region) and Prydz Bay in January 1985. Taxonomic identity, distribution and abundance data were obtained, together with an extensive range of pigment analysis, using high performance liquid chromatography (HPLC). Over 60 pigments were analysed (only the major ones are listed here). The major phytoplankton investigated were diatoms, dinoflagellates and flagellates. This dataset is a subset of the full cruise.

An excel spreadsheet containing the full pigment analysis obtained from the cruise is available for download from the URL given below. The spreadsheet is a digital version of the data presented in ANARE Research Notes 58, which was a report written based on this dataset. There are three worksheets to the spreadsheet:

1) Abbrev. - details the abbreviations used in worksheets 2 and 3.
2) Table 3 - Table 3 data entered from ANARE Research Notes 58.
3) Transposed Table 3 - The same data as worksheet 2, but arranged differently.

A pdf copy of ANARE Research Notes 58 is also available for download at the URL given below. A paper written in 2006 about pigments in microalgae, which provides some up-to-date explanations about pigmentation, is also available for download, but owing to copyright restrictions, is only available for download by Australian Antarctic Division personnel.

The fields in this dataset are:

Date
Time (GMT)
Latitude
Longitude
Depth (metres)
Pigment concentration (nanograms per litre)
chlorophyllide a
chlorophyll c
methyl chlorophyllide a
phaeophorbide a
peridinin
19'-butanoyloxyfucoxanthin
fucoxanthin
19'-hexanoyloxyfucoxanthin
Neoxanthin
Prasinoxanthin
Violaxanthin
Diadinoxanthin
Alloxanthin
diatoxanthin
Zeaxanthin
Canthaxanthin
Unknown
Chlorophyll b
Chlorophyll a allomer
Chlorophyll a
Chlorophyll a epimer
Phaeophytin a derivative
Phaeophytin b
Phaeophytin a
Chlorophyll a total
% Degradation
Pigment total

This work was completed as part of ASAC project 40 (ASAC_40).

Related URL
Link: View Related Information
Description: Download point for the excel spreadsheet


Link: View Related Information
Description: Download point for ANARE Research Notes 58


Link: View Related Information
Description: Download point for the 2006 Jeffrey and Wright Paper


Link: View Related Information
Description: Public information for ASAC project 40


Link: View Related Information
Description: Citation reference for this metadata record and dataset

Geographic Coverage
 N: -58.0 S: -70.0  E: 94.0  W: 57.0
 Min Depth: 0 METRES  Max Depth: 602 METRES

Data Set Citation
Dataset Creator: Wright, S.
Dataset Title: Nella Dan: SIBEX II Cruise Phytoplankton Data
Dataset Series Name: CAASM Metadata
Dataset Release Date: 2007-04-19
Dataset Publisher: Australian Antarctic Data Centre
Version: 1
Online Resource: https://data.aad.gov.au/metadata/records/SIBEX_II_phyto


Temporal Coverage
Start Date: 1985-01-03
Stop Date: 1985-01-26


Location Keywords
OCEAN > SOUTHERN OCEAN
GEOGRAPHIC REGION > POLAR


Science Keywords
BIOSPHERE >VEGETATION >CHLOROPHYLL    [Definition]
BIOLOGICAL CLASSIFICATION >PROTISTS >FLAGELLATES    [Definition]
BIOLOGICAL CLASSIFICATION >PROTISTS >FLAGELLATES >DINOFLAGELLATES    [Definition]
BIOLOGICAL CLASSIFICATION >PLANTS >MICROALGAE    [Definition]
BIOLOGICAL CLASSIFICATION >PLANTS >MICROALGAE >DIATOMS    [Definition]
BIOSPHERE >ECOSYSTEMS >AQUATIC ECOSYSTEMS >PLANKTON >PHYTOPLANKTON    [Definition]
OCEANS >OCEAN CHEMISTRY >PIGMENTS >CHLOROPHYLL    [Definition]
BIOLOGICAL CLASSIFICATION >PLANTS >MICROALGAE >DINOFLAGELLATES    [Definition]


Platform
SHIPS    [Information]


Instrument
CTD >Conductivity, Temperature, Depth    [Information]
HPLC >High-Performance Liquid Chromatograph    [Information]
NISKIN BOTTLES    [Information]


Quality
This voyage is one of six which studied the abundance and distribution of both phytoplankton and Euphausia superba. They provided a good coverage of the area at varying season times and conditions.

Data collection: Seven north-south transects were made between 58 degrees and 93 degrees east, with water samples collected at intervals of 5 degrees longitude. A General Oceanics rosette sampler equipped with twelve 5L Niskin botles was used for water collection in the top 200m of the water column. Standard depths for pigment analysis were used; 0, 10, 25, 35, 50, 75, 100, 200m. Temperature and salinity in the vertical profile were also measured, using a Neil Brown Mark 3 CTD.

Ship-board processing: The water samples were analysed for oxygen, salinity, nutrients, and phytoplankton species. The remaining 2L to 4L of the water sample were filtered under a slight vacuum (approx. 0.5 atm.) using Whatman GF/F filters (0.7 micro-metre nominal pore size, 47mm diameter), and the filters were then immediately frozen in liquid nitrogen.

Post-ship processing: The frozen filter was broken into 5mm diameter pieces, and sonicated in 4mL methanol using a Braun Labsonic 1510 sonicator equipped with a 4mm diameer needle probe (30 seconds at 50W). It was then filtered using a simple centrifugal system, described in Wright, S. and Shearer, J. (1984) Rapid extraction and high-performance liquid chromatography of chlorophylls and cartenoids from marine plankton. Journal of Chromatography 294:281-295. The filter debris was washed with 0.5mL methanol and recentrifuged. The combined extract was filtered through a Millex-HV filter unit (0.5 micro-metre pore size, Millipore Corp.). High performance liquid chromatography (HPLC) was used for pigment analysis. A 200 micro-litre sample of the extract was injected directly into a Waters Associates liquid chromatograph. Two RCM-100 radial compression modules were used in series. The pigments were eluted using a linear gradient from 90:10 acetonitrile:water to 100% ethyl acetate over 20 minutes at 2mL/min. They were detected using a Waters 440 absorbance detector and integrated using a Waters Data Module. Small peak heights were measured manually and converted to pigment abundance using individual calibration curves, as the Data Module was found to be inaccurate when the signal-to-noise ratio was low. Absorption spectra were taken using a Hewlett-Packard 8450A spectrophotometer.

Data are logically consistent, as oceanographic data (CTD measurements) were also obtained at the same sampling sites as the phytoplankton water collection sites. Large variations were found in the vertical distribution of the marker pigments along each north-south transect. In the major pigments, this represented patchiness in the distribution of phytoplankton in the water column, as the accuracy of the HPLC technique is within 5%. In minor pigments, experimental errors would have been more significant, and would have been superimposed on this patchiness.

These data were manually typed in from ANARE Research Notes 58 by AADC staff. It is possible that some typing errors occurred. Several inconsitencies in the printed data were also discovered.


Access Constraints
An excel spreadsheet of data are available for download from the URL given below. A pdf copy of ANARE Research Notes 58 is also available for download from the URL given below.

A pdf copy of a 2006 paper detailing pigmentation is available for download by Australian Antarctic Division personnel only.


Use Constraints
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIBEX_II... when using these data.


Keywords
Date
Time
Latitude
Longitude
Depth
Pigment concentration
chlorophyllide a
chlorophyll c
methyl chlorophyllide a
phaeophorbide a
peridinin
19'-butanoyloxyfucoxanthin
fucoxanthin
19'-hexanoyloxyfucoxanthin
Prasinoxanthin
Neoxanthin
Diadinoxanthin
Violaxanthin
Alloxanthin
diatoxanthin
Zeaxanthin
Canthaxanthin
Unknown
Chlorophyll b
Chlorophyll a allomer
Chlorophyll a
Chlorophyll a epimer
Phaeophytin a derivative
Phaeophytin b
Phaeophytin a
Chlorophyll a total
% Degradation
Pigment total


Data Set Progress
COMPLETE


Originating Center
Australian Antarctic Division


Data Center
Australian Antarctic Data Centre, Australia    [Information]
Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia



Distribution
Distribution_Media: HTTP
Distribution_Size: 142 kb
Distribution_Format: Excel
Fees: free

Distribution_Media: HTTP
Distribution_Size: 10.4 MB
Distribution_Format: pdf
Fees: free

Distribution_Media: HTTP
Distribution_Size: 4.4 MB
Distribution_Format: pdf
Fees: free


Personnel
SIMON WRIGHT
Role: INVESTIGATOR
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia


Publications/References
Wright, S.W. (1987), Phytoplankton pigment data: Prydz Bay region - SIBEX II, MV Nella Dan , January 1985., ANARE Research Notes, 58, 102

Jeffrey, S.W., Wright, S.W. (2006), Photosynthetic pigments in marine microalgae: Insights from cultures and the sea., 34-90, In: Subba Rao, D.V. (ed.) Algal Cultures, Analogues of Blooms and Applications
Extended Metadata Properties
(Click to view more)

Creation and Review Dates
DIF Creation Date: 1999-10-07
Last DIF Revision Date: 2019-03-20



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