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Antarctic diatom silicification diminishes under ocean acidification
Entry ID: AAS_4026_Silicification_CO2


Summary
Abstract: This data set was collected during an ocean acidification mesocosm experiment performed at Davis Station, Antarctica during the 2014/15 summer season. It includes:
- description of methods for all data collection and analyses.
- diatom cell volume
- bulk silicification
- species specific silicification via fluorescence microscopy
- bulk community Fv/Fm on day 12
- single-cell PAM fluorometry data (maximum quantum yield of PSII: Fv/Fm)

A natural community of Antarctic marine microbes from Prydz Bay, East Antarctica were exposed to a range of CO2 concentrations in 650 L minicosms to simulate possible future ocean conditions up to the year ~2200. Diatom silica precipitation rates were examined at CO2 concentrations between 343 to 1641 micro atm, measuring both the total diatom community response and that of individual species, to determine whether ocean acidification may influence future diatom ballast and therefore alter carbon and silica fluxes in the Southern Ocean.

Described and analysed in:
Antarctic diatom silicification diminishes under ocean acidification (submitted for review)

Methods described in:
Antarctic diatom silicification diminishes under ocean acidification (submitted for review)

Location: Prydz bay, Davis Station, Antarctica (68 degrees 35'S, 77 degrees 58' E)
Date: Summer 2014/2015

Worksheet descriptions:

Bulk silicification - raw data
Measured total and incorporated biogenic silica using spectrophotometer for all tanks on day 12 after 24 h incubation with PDMPO - raw data

Bulk Fv/Fm - dark-adapted maximum quantum efficiency of PSII (Fv/Fm) on whole community - raw data
Measured Fv/Fm of individual cells from 3 mesocosm tanks.

Single-cell silicificiation, Fluorescence microscopy - raw data
Measured autofluorescence and PDMPO fluorescence of individual diatoms from 6 mesocosm tanks

Single-cell PAM, dark-adapted maximum quantum efficiency of PSII (Fv/Fm) - raw data
Measured Fv/Fm of individual cells from 3 mesocosm tanks.

Cell volume
Calculated cell volume (um3) of 7 species from minicosm tanks 1 and 6 - raw data


Abbreviations:
Fv/Fm Maximum quantum yield of PSII
PDMPO 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole
Tant Thalassiosira antarctica
DiscLg Large Discoid centric diatoms
Stella Stellarima microtrias
Chaeto Chaetoceros spp.
Prob Proboscia truncata
Pseu Pseudonitzschia turgiduloides
FragLg Fragilariopsis cylindrus / curta
Centric Large Discoid centric diatoms
LargeThalassiosira Large Discoid centric diatoms


Purpose: Based on the results of a previous study, a natural community of Antarctic marine microbes from Prydz Bay, East Antarctica were exposed to a range of CO2 concentrations in 650 L mesocosms to simulate possible future ocean conditions up to the year ?2200. Diatom silica precipitation rates were examined at CO2 concentrations between 343 to 1641 micro atm, measuring both the total diatom community response and that of individual species, to determine whether ocean acidification may influence future diatom ballast and therefore alter carbon and silica fluxes in the Southern Ocean.

Related URL
Link: View Related Information
Description: Citation reference for this metadata record and dataset.


Link: View Related Information
Description: Public information for AAS project AAS_4026


Link: View Related Information
Description: Download the dataset

Parent DIF
This description is a member of a collection. The collection is described in
AAS_4026_Ocean_Acidification_Marine_Microbes_Parent


Geographic Coverage
 N: -67.38509 S: -69.79988  E: 82.69531  W: 73.75

Data Set Citation
Dataset Creator: Petrou, K.
Dataset Title: Antarctic diatom silicification diminishes under ocean acidification
Dataset Series Name: CAASM Metadata
Dataset Release Date: 2019-04-30
Dataset Publisher: Australian Antarctic Data Centre
Version: 1
Online Resource: https://data.aad.gov.au/metadata/records/AAS_4026_Silicification_CO2


Temporal Coverage
Start Date: 2014-11-19
Stop Date: 2015-01-14

Start Date: 2017-06-01
Stop Date: 2018-06-30


Location Keywords
GEOGRAPHIC REGION > POLAR
CONTINENT > ANTARCTICA > DAVIS STATION
OCEAN > SOUTHERN OCEAN > PRYDZ BAY


Science Keywords
BIOLOGICAL CLASSIFICATION >PLANTS >MICROALGAE >DIATOMS    [Definition]
OCEANS >OCEAN CHEMISTRY >SILICATE    [Definition]


Platform
FIELD INVESTIGATION    [Information]
FIELD SURVEYS    [Information]
LABORATORY    [Information]


Instrument
SPECTROPHOTOMETERS    [Information]
PAM >PORTABLE FLUORESCENCE ANALYZERS    [Information]
FLUOROMETERS    [Information]
FLUORESCENCE MICROSCOPY    [Information]


Quality
No problems were encountered with data collection. The six level fCO2 gradient approach meant that our data could be analysed using a regression model, allowing us to identify functional relationships between our fCO2 treatment and our response variables. Gradient designs are more effective at uncovering underlying responses patterns to environmental drivers, improving interpolation potential and generally deliver more useful quantitative information for models.


Access Constraints
These data are not yet publicly available.


Use Constraints
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4026... when using these data.


Keywords
SILICIFICATION
DIATOMS
CARBON CYCLING
OCEAN ACIDIFICATION
MESOCOSM
MINICOSM


Data Set Progress
COMPLETE


Originating Center
Australian Antarctic Division


Data Center
Australian Antarctic Data Centre, Australia    [Information]
Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia



Distribution
Distribution_Media: HTTP
Distribution_Size: 152 kb
Distribution_Format: Excel
Fees: Free


Personnel
KATHERINA PETROU
Role: INVESTIGATOR
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Email: katherina.petrou at uts.edu.au
Contact Address:
15 Broadway
City: Ultimo
Province or State: New South Wales (NSW)
Postal Code: 2007
Country: Australia


Extended Metadata Properties
(Click to view more)

Creation and Review Dates
DIF Creation Date: 2019-01-15
Last DIF Revision Date: 2019-01-16



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