X-ray diffraction data using ice-binding protein (LeIBP) crystalEntry ID: KPDC_X-rayDUIB_LeIBP_2012
Abstract: Psychrophilic Arctic yeast Leucosporidium sp. produces a glycosylated ice-binding protein (LeIBP) with a molecular mass of approximately 25 kDa, which can lower the freezing point below the melting point once it binds to ice. LeIBP exhibits low amino acid sequence similarity to other antifreeze proteins with known protein structures. Recently, we developed an expression system allowing high-level production and efficient purification of recombinant pLeIBP. Furthermore, crystallization and preliminary X-ray crystallographic analysis of the ice-binding protein were performed.
Purpose: To investigate the antifreeze mechanism of LeIBP, we have carried out structural studies. As the first step toward its structural elucidation, we report the results of preliminary X-ray crystallographic experiments with LeIBP.
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Role: DIF AUTHOR
Role: TECHNICAL CONTACT
Email: junhyucklee at kopri.re.kr
Songdo Techno Park, 7-50, Songdo-dong, Yeonsu-gu
Postal Code: 406-840
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Creation and Review Dates
DIF Creation Date: 2013-05-06
Last DIF Revision Date: 2017-08-24