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Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses

Abstract: Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats.

This work was completed as part of ASAC project 2899 (ASAC_2899).
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Geographic Coverage
Spatial coordinates   
  N: -46.0   S: -67.0   E: 150.0   W: 140.0

Data Set Citation
Dataset Originator/Creator: Cavicchioli, R., Hoffman, J. and McQuaid, J.
Dataset Title: Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses
Dataset Series Name: CAASM Metadata
Dataset Release Date: 2012-04-24
Dataset Publisher: Australian Antarctic Data Centre
Online Resource:
Temporal Coverage
Start Date: 2007-12-17
Stop Date: 2008-01-28
Quality No known quality issues.

GOS stands for Global Ocean Sampling.
TFF- Tangential Flow Filtration.
Access Constraints These data are publicly available from the provided URL.
Use Constraints This data set conforms to the PICCCBY Attribution License

Please follow instructions listed in the citation reference provided at when using these data.
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Data Center
Australian Antarctic Data Centre, Australia Supplemental Info
Data Center URL:

Data Center Personnel
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia
Distribution Media: HTTP
Fees: free
Phone: +61 2 9385 3516
Email: r.cavicchioli at
Contact Address:
School Of Microbiology and Immunology
University Of New South Wales
City: Sydney
Province or State: NSW
Postal Code: 2052
Country: Australia
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Creation and Review Dates
DIF Creation Date: 2008-02-24
Last DIF Revision Date: 2017-09-11