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Fluorescence illumination and observation is the most rapidly
expanding microscopy technique employed today, both in the
medical and biological sciences, a fact which has spurred the
development of more sophisticated microscopes and numerous
fluorescence accessories. Epi-fluorescence, or incident light
fluorescence, has now become the method of choice in many
applications and comprises a large part of this tutorial. We
have divided the fluorescence section of the primer into several
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